TY - JOUR T1 - Circlator: automated circularization of genome assemblies using long sequencing reads JF - bioRxiv DO - 10.1101/023408 SP - 023408 AU - Martin Hunt AU - Nishadi De Silva AU - Thomas D Otto AU - Julian Parkhill AU - Jacqueline A Keane AU - Simon R Harris Y1 - 2015/01/01 UR - http://biorxiv.org/content/early/2015/07/30/023408.abstract N2 - The assembly of DNA sequence data into finished genomes is undergoing a renais-sance thanks to emerging technologies producing reads of tens of kilobases. Assembling complete bacterial and small eukaryotic genomes is now possible, but the final step of circularizing sequences remains unsolved. Here we present Circlator, the first tool to automate assembly circularization and produce accurate linear rep-resentations of circular sequences. Using Pacific Biosciences and Oxford Nanopore data, Circlator correctly circularized 26 of 27 circularizable sequences, comprising 11 chromosomes and 12 plasmids from bacteria, the apicoplast and mitochondrion of Plasmodium falciparum and a human mitochondrion. Circlator is available at http://sanger-pathogens.github.io/circlator/.NCTCNational Collection of Type CulturesONTOxford Nanopore TechnologiesPacBioPacific Biosciences ER -