RT Journal Article
SR Electronic
T1 Differential stoichiometry among core ribosomal proteins
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 005553
DO 10.1101/005553
A1 Nikolai Slavov
A1 Stefan Semrau
A1 Edoardo Airoldi
A1 Bogdan A. Budnik
A1 Alexander van Oudenaarden
YR 2015
UL http://biorxiv.org/content/early/2015/07/20/005553.abstract
AB Understanding the regulation and structure of ribosomes is essential to under-standing protein synthesis and its deregulation in disease. While ribosomes are believed to have a fixed stoichiometry among their core ribosomal proteins (RPs), some experiments suggest a more variable composition. Testing such variability requires direct and precise quantification of RPs. We used mass-spectrometry to directly quantify RPs across monosomes and polysomes of mouse embryonic stem cells (ESC) and budding yeast. Our data show that the stoichiometry among core RPs in wild-type yeast cells and ESC depends both on the growth conditions and on the number of ribosomes bound per mRNA. Furthermore, we find that the fitness of cells with a deleted RP-gene is inversely proportional to the enrichment of the corresponding RP in polysomes. Together, our findings support the existence of ribosomes with distinct protein composition and physiological function.