PT - JOURNAL ARTICLE AU - Bo Lu AU - Stephen Fleming AU - Tamas Szalay AU - Jene Golovchenko TI - Thermal motion of DNA in an MspA pore AID - 10.1101/021766 DP - 2015 Jan 01 TA - bioRxiv PG - 021766 4099 - http://biorxiv.org/content/early/2015/06/30/021766.short 4100 - http://biorxiv.org/content/early/2015/06/30/021766.full AB - We report on an experiment and calculations that determine the thermal motion of a voltage-clamped ssDNA-NeutrAvidin complex in an MspA nanopore. The electric force and diffusion constant of DNA inside an MspA pore have been determined in order to evaluate DNA’s thermal position fluctuations. We show that an out-of-equilibrium state returns to equilibrium so quickly that experiments usually measure a weighted average over the equilibrium position distribution. Averaging over the equilibrium position distribution is consistent with results of state-of-the-art nanopore sequencing experiments. It is shown that a reduction in thermal averaging can be achieved by increasing the electrophoretic force used in nanopore sequencing devices.