RT Journal Article SR Electronic T1 A microRNA profile in Fmr1 knockout mice reveals microRNA expression alterations with possible roles in fragile X syndrome JF bioRxiv FD Cold Spring Harbor Laboratory SP 002071 DO 10.1101/002071 A1 Ting Liu A1 Rui-Ping Wan A1 Ling-Jia Tang A1 Shu-Jing Liu A1 Hai-Jun Li A1 Qi-Hua Zhao A1 Wei-Ping Liao A1 Xiao-Fang Sun A1 Yong-Hong Yi A1 Yue-Sheng Long YR 2014 UL http://biorxiv.org/content/early/2014/01/26/002071.abstract AB Fragile X syndrome (FXS), a common form of inherited mental retardation, is caused by a loss of expression of the fragile X mental retardation protein (FMRP). FMRP is involved in brain functions by interacting with mRNAs and microRNAs (miRNAs) that selectively control gene expression at translational level. However, little is known about the role of FMRP in regulating miRNA expression. Here, we found a development-dependant dynamic expression of Fmr1 mRNA (encoding FMRP) in mouse hippocampus with a small peak at postnatal day 7 (P7). MiRNA microarray analysis showed that the levels of 38 miRNAs showed a significant increase with about 15∼250 folds and the levels of 26 miRNAs showed a significant decrease with only about 2∼4 folds in the hippocampus of P7 Fmr1 KO mice. Q-PCR assay showed that 9 of the most increased miRNAs (>100 folds in microarrays) were increased about 40∼70 folds and their pre-miRNAs were increased about 5∼10 folds, but no significant difference in their pri-miRNA levels was observed, suggesting a role of FMRP in regulating miRNA processing from pri-miRNA to pre-miRNA. We further demonstrated that a set of protein-coding mRNAs, potentially targeted by the 9 miRNAs were down-regulated in the hippocampus of Fmr1 KO mice. Finally, luciferase assays demonstrated that miR-34b, miR-340, miR-148a could down-regulate the reporter gene expression by interacting with the Met 3′ UTR. Taken together these findings suggest that the miRNA expression alterations resulted from the absence of FMRP might contribute to molecular pathology of FXS.