RT Journal Article
SR Electronic
T1 Subcellular compartments interplay for carbon and nitrogen allocation in <em>Chromera velia</em> and <em>Vitrella brassicaformis</em>
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 603555
DO 10.1101/603555
A1 Zoltán Füssy
A1 Tereza Faitová
A1 Miroslav Oborník
YR 2019
UL http://biorxiv.org/content/early/2019/04/09/603555.abstract
AB Endosymbioses necessitate functional cooperation of cellular compartments to avoid pathway redundancy and streamline the control of biological processes. To gain insight into the metabolic compartmentation in chromerids, phototrophic relatives to apicomplexan parasites, we prepared a reference set of proteins probably localized to mitochondria, cytosol and the plastid, taking advantage of available genomic and transcriptomic data. Training of prediction algorithms with the reference set now allows a genome-wide analysis of protein localization in C. velia and V. brassicaformis. We found that the chromerid plastids house enzymatic pathways needed for their maintenance and photosynthetic activity, but for carbon and nitrogen allocation, metabolite exchange is necessary with the cytosol and mitochondria. This indeed suggests that the regulatory mechanisms operate in the cytosol to control carbon metabolism based on the availability of both light and nutrients. We discuss that this arrangement is largely shared with apicomplexans, possibly stemming from a common ancestral metabolic architecture, and supports the mixotrophy of the chromerid algae.2-OG2-oxoglutarateACPacyl-carrier proteinALAdelta-aminolevulinic acidBTSbipartite (plastid) targeting signalChl achlorophyll aDGDG/MGDGdi-/monogalactosyl-diacylglycerolipidDHAPdihydroxyacetone phosphateEry-4Perythrose 4-phosphateFADflavin adenine dinucleotideGS/GOGATglutamine synthase / glutamine oxoglutarate aminotransferaseIMSinter-membrane spaceMEPmethyl-D-erythritol 4-phosphatemTPmitochondrial transit peptideOAAoxaloacetateornornithinePEPphosphoenolpyruvatePQplastoquinolPRPP5-phosphoribosyl-1-pyrophosphatepyrpyruvateRu-5Pribulose 5-phosphateTCAtricarboxylic acidTHFtetrahydrofolateUQubiquinone.