PT - JOURNAL ARTICLE AU - Rowena Ball AU - John Brindley TI - Thiosulfate-hydrogen peroxide redox oscillator as pH driver for ribozyme activity in the RNA world AID - 10.1101/020693 DP - 2015 Jan 01 TA - bioRxiv PG - 020693 4099 - http://biorxiv.org/content/early/2015/06/10/020693.short 4100 - http://biorxiv.org/content/early/2015/06/10/020693.full AB - The RNA world of more than 3.7 billion years ago may have drawn on thermal and pH oscillations set up by the oxidation of thiosulfate by hydrogen peroxide (the THP oscillator) as a power source to drive replication. Since this primordial RNA also must have developed enzyme functionalities, in this work we examine the responses of two simple ribozymes to a THP periodic drive, using experimental rate and thermochemical data in a dynamical model for the coupled, self-consistent evolution of all reactants and intermediates. The resulting time traces show that ribozyme performance can be enhanced under pH cycling, and that thermal cycling may have been necessary to achieve large performance gains. We discuss three important ways in which the dynamic hydrogen peroxide medium may have acted as an agent for development of the RNA world towards a cellular world: proton gradients, resolution of the ribozyme versus replication paradox, and vesicle formation.