RT Journal Article
SR Electronic
T1 <em>Ex vivo</em> mycobacterial growth inhibition assay (MGIA) for tuberculosis vaccine testing - a protocol for mouse splenocytes
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 020560
DO 10.1101/020560
A1 Andrea Zelmer
A1 Rachel Tanner
A1 Elena Stylianou
A1 Sheldon Morris
A1 Angelo Izzo
A1 Ann Williams
A1 Sally Sharpe
A1 Ilaria Pepponi
A1 Barry Walker
A1 David A. Hokey
A1 Helen McShane
A1 Michael Brennan
A1 Helen Fletcher
YR 2015
UL http://biorxiv.org/content/early/2015/06/07/020560.abstract
AB The testing of vaccines for tuberculosis is costly and time-consuming, and dependent on preclinical animal challenge models and clinical trials. We have recently developed a mycobacterial growth inhibition assay (MGIA) to test vaccine efficacy ex vivo. This assay measures the summative effect of the host immune response and may serve as a novel tool to facilitate vaccine testing. It has generated much interest recently, and to facilitate technology transfer and reproducibility between laboratories, we here describe a detailed protocol for an ex vivo MGIA in mouse splenocytes.