%0 Journal Article %A Andrea Zelmer %A Rachel Tanner %A Elena Stylianou %A Sheldon Morris %A Angelo Izzo %A Ann Williams %A Sally Sharpe %A Ilaria Pepponi %A Barry Walker %A David A. Hokey %A Helen McShane %A Michael Brennan %A Helen Fletcher %T Ex vivo mycobacterial growth inhibition assay (MGIA) for tuberculosis vaccine testing - a protocol for mouse splenocytes %D 2015 %R 10.1101/020560 %J bioRxiv %P 020560 %X The testing of vaccines for tuberculosis is costly and time-consuming, and dependent on preclinical animal challenge models and clinical trials. We have recently developed a mycobacterial growth inhibition assay (MGIA) to test vaccine efficacy ex vivo. This assay measures the summative effect of the host immune response and may serve as a novel tool to facilitate vaccine testing. It has generated much interest recently, and to facilitate technology transfer and reproducibility between laboratories, we here describe a detailed protocol for an ex vivo MGIA in mouse splenocytes. %U https://www.biorxiv.org/content/biorxiv/early/2015/06/07/020560.full.pdf