TY - JOUR T1 - <em>Ex vivo</em> mycobacterial growth inhibition assay (MGIA) for tuberculosis vaccine testing - a protocol for mouse splenocytes JF - bioRxiv DO - 10.1101/020560 SP - 020560 AU - Andrea Zelmer AU - Rachel Tanner AU - Elena Stylianou AU - Sheldon Morris AU - Angelo Izzo AU - Ann Williams AU - Sally Sharpe AU - Ilaria Pepponi AU - Barry Walker AU - David A. Hokey AU - Helen McShane AU - Michael Brennan AU - Helen Fletcher Y1 - 2015/01/01 UR - http://biorxiv.org/content/early/2015/06/07/020560.abstract N2 - The testing of vaccines for tuberculosis is costly and time-consuming, and dependent on preclinical animal challenge models and clinical trials. We have recently developed a mycobacterial growth inhibition assay (MGIA) to test vaccine efficacy ex vivo. This assay measures the summative effect of the host immune response and may serve as a novel tool to facilitate vaccine testing. It has generated much interest recently, and to facilitate technology transfer and reproducibility between laboratories, we here describe a detailed protocol for an ex vivo MGIA in mouse splenocytes. ER -