TY - JOUR T1 - Myc-induced cell mixing is required for competitive tissue invasion and destruction JF - bioRxiv DO - 10.1101/020313 SP - 020313 AU - Romain Levayer AU - Barbara Hauert AU - Eduardo Moreno Y1 - 2015/01/01 UR - http://biorxiv.org/content/early/2015/06/02/020313.abstract N2 - Cell-cell intercalation is used in several developmental processes to shape the normal body plan1. There is no clear evidence that intercalation is involved in pathologies. Here, we use the proto-oncogene Myc to study a process analogous to early phase of tumour expansion: Myc-induced cell competition2-7. Cell competition is a conserved mechanism5,6,8,9 driving the elimination of slow proliferating cells (so called losers) by faster proliferating neighbours (so called winners) through apoptosis10 and is important to prevent developmental malformations and maintain tissue fitness11. Using long term live imaging of Myc-driven competition in the Drosophila pupal notum and in the wing imaginal disc, we show that the probability of elimination of loser cells correlates with the surface of contact shared with winners. As such, modifying loser/winner interface morphology can modulate the strength of competition. We further show that elimination of loser clones requires winner/loser cell mixing through cell-cell intercalation. Cell mixing is driven by differential growth and the high tension at winner-winner interfaces relative to winner-loser and loser-loser interfaces, which leads to a preferential stabilisation of winner-loser contacts and reduction of clone compactness over time. Differences in tension are generated by a relative difference of junctional F-actin levels between loser and winner junctions, induced by differential levels of the phosphatidylinositol PIP3. Our results establish the first link between cell-cell intercalation induced by a proto-oncogene and how it promotes invasiveness and destruction of healthy tissues. ER -