RT Journal Article
SR Electronic
T1 Extensive post-transcriptional regulation across human tissues
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 020206
DO 10.1101/020206
A1 Nikolai Slavov
A1 Alexander Franks
A1 Edoardo Airoldi
YR 2015
UL http://biorxiv.org/content/early/2015/05/31/020206.abstract
AB Transcriptional and post-transcriptional regulation shape tissue-type-specific proteomes, but their relative contributions remain contested. Common estimates of the factors determining protein levels do not distinguish between (i) the factors determining the variability between the abundances of different proteins and (ii) the factors determining the physiological variability of the same protein across different tissue types. We estimated the factors determining these two orthogonal sources of variability and found that the variability between the levels of different proteins is dominated by transcriptional regulation while in contrast the physiological variability across tissue-types is dominated by post-transcriptional regulation. These results reconcile existing estimates in the liter ature, caution against estimating protein fold-changes from mRNA fold-changes between different cell-types, and highlight the contribution of post-transcriptional regulation in shaping tissue-type-specific proteomes.