RT Journal Article
SR Electronic
T1 Discovery of <em>Pandoraea pnomenusa</em> RB38 <em>N</em>-acyl homoserine Lactone Synthase (PpnI) and its Complete Genome Sequence Analysis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 020180
DO 10.1101/020180
A1 Kok-Gan Chan
A1 Robson Ee
A1 Kah-Yan How
A1 Siew-Kim Lee
A1 Wai-Fong Yin
A1 Yan-Lue Lim
YR 2015
UL http://biorxiv.org/content/early/2015/05/31/020180.abstract
AB In this study, we sequenced the genome of P. pnomenusa RB38 and reported the finding of a pair of cognate luxI/R homologs which we firstly coined as ppnI, which is found adjacent to a luxR homolog, ppnR. An additional orphan luxR homolog, ppnR2 was also discovered. Multiple sequence alignment revealed that PpnI is a distinct cluster of AHL synthase compared to those of its nearest phylogenetic neighbor, Burkholderia spp. When expressed heterologously and analysed using high resolution tandem mass spectrometry, PpnI directs the synthesis of N-octanoylhomoserine lactone (C8-HSL). To our knowledge, this is the first documentation of the luxI/R homologs of the genus of Pandoraea.