RT Journal Article
SR Electronic
T1 Determining Exon Connectivity in Complex mRNAs by Nanopore Sequencing
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 019752
DO 10.1101/019752
A1 Mohan T. Bolisetty
A1 Gopinath Rajadinakaran
A1 Brenton R. Graveley
YR 2015
UL http://biorxiv.org/content/early/2015/05/22/019752.abstract
AB Though powerful, short-read high throughput RNA sequencing is limited in its ability to directly measure exon connectivity in mRNAs containing multiple alternative exons located farther apart than the maximum read lengths. Here, we use the Oxford Nanopore MinION™ sequencer to identify 7,899 ‘full-length’ isoforms expressed from four Drosophila genes, Dscam1, MRP, Mhc, and Rdl. These results demonstrate that nanopore sequencing can be used to deconvolute individual isoforms and that it has the potential to be an important method for comprehensive transcriptome characterization.