PT  - JOURNAL ARTICLE
AU  - Mohan T. Bolisetty
AU  - Gopinath Rajadinakaran
AU  - Brenton R. Graveley
TI  - Determining Exon Connectivity in Complex mRNAs by Nanopore Sequencing
AID  - 10.1101/019752
DP  - 2015 Jan 01
TA  - bioRxiv
PG  - 019752
4099  - http://biorxiv.org/content/early/2015/05/22/019752.short
4100  - http://biorxiv.org/content/early/2015/05/22/019752.full
AB  - Though powerful, short-read high throughput RNA sequencing is limited in its ability to directly measure exon connectivity in mRNAs containing multiple alternative exons located farther apart than the maximum read lengths. Here, we use the Oxford Nanopore MinION™ sequencer to identify 7,899 ‘full-length’ isoforms expressed from four Drosophila genes, Dscam1, MRP, Mhc, and Rdl. These results demonstrate that nanopore sequencing can be used to deconvolute individual isoforms and that it has the potential to be an important method for comprehensive transcriptome characterization.