TY - JOUR T1 - Determining Exon Connectivity in Complex mRNAs by Nanopore Sequencing JF - bioRxiv DO - 10.1101/019752 SP - 019752 AU - Mohan T. Bolisetty AU - Gopinath Rajadinakaran AU - Brenton R. Graveley Y1 - 2015/01/01 UR - http://biorxiv.org/content/early/2015/05/22/019752.abstract N2 - Though powerful, short-read high throughput RNA sequencing is limited in its ability to directly measure exon connectivity in mRNAs containing multiple alternative exons located farther apart than the maximum read lengths. Here, we use the Oxford Nanopore MinION™ sequencer to identify 7,899 ‘full-length’ isoforms expressed from four Drosophila genes, Dscam1, MRP, Mhc, and Rdl. These results demonstrate that nanopore sequencing can be used to deconvolute individual isoforms and that it has the potential to be an important method for comprehensive transcriptome characterization. ER -