RT Journal Article
SR Electronic
T1 Real-time strain typing and analysis of antibiotic resistance potential using Nanopore MinION sequencing
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 019356
DO 10.1101/019356
A1 Minh Duc Cao
A1 Devika Ganesamoorthy
A1 Alysha G. Elliott
A1 Huihui Zhang
A1 Matthew A. Cooper
A1 Lachlan Coin
YR 2015
UL http://biorxiv.org/content/early/2015/05/20/019356.abstract
AB Clinical pathogen sequencing has significant potential to drive informed treatment of patients with unknown bacterial infection. However, the lack of rapid sequencing technologies with concomitant analysis has impeded clinical adoption in infection diagnosis. Here we demonstrate that commercially-available Nanopore sequencing devices can identify bacterial species and strain information with less than one hour of sequencing time, initial drug-resistance profiles within 2 hours, and a complete resistance profile within 12 hours. We anticipate these devices and associated analysis methods may become useful clinical tools to guide appropriate therapy in time-critical clinical presentations such as bacteraemia and sepsis.