RT Journal Article
SR Electronic
T1 Two-Dimensional Mapping of 3D Data from Confocal Microscopy
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 019133
DO 10.1101/019133
A1 Anthony Fan
A1 Justin Cassidy
A1 Richard W. Carthew
A1 Sascha Hilgenfeldt
YR 2015
UL http://biorxiv.org/content/early/2015/05/08/019133.abstract
AB Confocal microscopy has been experimentally proven for decades to provide high-quality images for biological research. Its unique property of blocking out-of-focus light enables 3D rendering from planar stacks and visualization of internal features. However, visualizing 3D data on a flat display is not intuitive, and would lead to occasional distortion. In this study, a novel, easy-to-implement, and computationally fast solution is provided to reconstruct a confocal stack to true 3D data and subsequently map the information correctly with size and shape consistency to a 2D space for visualization and image analysis purposes.