RT Journal Article
SR Electronic
T1 NCBI BLAST + integrated into Galaxy
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 014043
DO 10.1101/014043
A1 Peter J. A. Cock
A1 John M. Chilton
A1 Björn Grüning
A1 James E. Johnson
A1 Nicola Soranzo
YR 2015
UL http://biorxiv.org/content/early/2015/05/04/014043.abstract
AB Background The NCBI BLAST suite has become ubiquitous in modern molecular biology, used for small tasks like checking capillary sequencing results of single PCR products through to genome annotation or even larger scale pan-genome analyses. For early adopters of the Galaxy web-based biomedical data analysis platform, integrating BLAST was a natural step for sequence comparison workflows.Findings The command line NCBI BLAST+ tool suite was wrapped for use within Galaxy, defining appropriate datatypes as needed, with the goal of making common BLAST tasks easy, and advanced tasks possible.Conclusions This effort has been come an informal international collaborative effort, and is deployed and used on Galaxy servers worldwide. Several example use-cases are described herein.