RT Journal Article
SR Electronic
T1 High Frequency Actionable Pathogenic Exome Mutations in an Average-Risk Cohort
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 151225
DO 10.1101/151225
A1 Shannon Rego
A1 Orit Dagan-Rosenfeld
A1 Wenyu Zhou
A1 M. Reza Sailani
A1 Patricia Limcaoco
A1 Elizabeth Colbert
A1 Monika Avina
A1 Jessica Wheeler
A1 Colleen Craig
A1 Denis Salins
A1 Hannes L. Röst
A1 Jessilyn Dunn
A1 Tracey McLaughlin
A1 Lars M. Steinmetz
A1 Jonathan A. Bernstein
A1 Michael P. Snyder
YR 2017
UL http://biorxiv.org/content/early/2017/06/18/151225.abstract
AB Whole exome sequencing (WES) is increasingly utilized in both clinical and non-clinical settings, but little is known about the utility of WES in healthy individuals. In order to determine the frequency of both medically actionable and non-actionable but medically relevant exome findings in the general population we assessed the exomes of 70 participants who have been extensively characterized over the past several years as part of a longitudinal integrated multi-omics profiling study at Stanford University. We assessed exomes for rare likely pathogenic and pathogenic variants in genes associated with Mendelian disease in the Online Mendelian Inheritance in Man (OMIM) database. We used American College of Medical Genetics (ACMG) guidelines were used for the classification of rare sequence variants, and additionally we assessed pharmacogenetic variants. Twelve out of 70 (17%) participants had medically actionable findings in Mendelian disease genes, including 6 (9%) with mutations in genes not currently included in the ACMG’s list of 59 actionable genes. This number is higher than that reported in previous studies and suggests added benefit from utilizing expanded gene lists and manual curation to assess actionable findings. A total of 60 participants (89%) had non-actionable findings identified including 57 who were found to be mutation carriers for recessive diseases and 21 who have increased Alzheimer’s disease risk due to heterozyg ous or homozygous APOE e4 alleles (18 participants had both). These results suggest that exome sequencing may have considerably more utility for health management in the general population than previously thought.