RT Journal Article
SR Electronic
T1 Post-exercise cold-water immersion increases Na<sup>+</sup>,K<sup>+</sup>-ATPase α<sub>2</sub>-isoform mRNA content in parallel with elevated Sp1 expression in human skeletal muscle
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 151100
DO 10.1101/151100
A1 Danny Christiansen
A1 Robyn M. Murphy
A1 James R. Broatch
A1 Jens Bangsbo
A1 Michael J. McKenna
A1 Jujiao Kuang
A1 David J. Bishop
YR 2017
UL http://biorxiv.org/content/early/2017/06/16/151100.abstract
AB We investigated the effect of a session of sprint-interval exercise on the mRNA content of NKA isoforms (α1-3, β1-3) and FXYD1 in human skeletal muscle. To explore some of the cellular stressors involved in this regulation, we evaluated the association between these mRNA responses and those of the transcription factors Sp1, Sp3 and HIF-1α. Given cold exposure perturbs muscle redox homeostasis, which may be one mechanism important for increases in NKA-isoform mRNA, we also explored the effect of post-exercise cold-water immersion (CWI) on the mRNA responses. Muscle was sampled from nineteen men before (Pre) and after (+0h, +3h) exercise plus passive rest (CON, n=10) or CWI (10°C; COLD, n=9). In COLD, exercise increased NKAα2 and Sp1 mRNA (+0h, p&lt;0.05). These genes remained unchanged in CON (p&gt;0.05). In both conditions, exercise increased NKAα1, NKAβ3 and HIF-1α mRNA (+3h; p &lt;0.05), decreased NKAβ2 mRNA (+3h; p&lt;0.05), whereas NKAα3, NKAβ1, FXYD1 and Sp3 mRNA remained unchanged (p&gt;0.05). These human findings highlight 1) sprint-interval exercise increases the mRNA content of NKA α1 and β3, and decreases that of NKA β2, which may relate, in part, to exercise-induced muscle hypoxia, and 2) post-exercise CWI augments NKAα2 mRNA, which may be associated with promoted Sp1 activation.