RT Journal Article SR Electronic T1 High-throughput annotation of full-length long noncoding RNAs with Capture Long-Read Sequencing (CLS) JF bioRxiv FD Cold Spring Harbor Laboratory SP 105064 DO 10.1101/105064 A1 Julien Lagarde A1 Barbara Uszczynska-Ratajczak A1 Silvia Carbonell A1 Sílvia Pérez-Lluch A1 Amaya Abad A1 Carrie Davis A1 Thomas R. Gingeras A1 Adam Frankish A1 Jennifer Harrow A1 Roderic Guigo A1 Rory Johnson YR 2017 UL http://biorxiv.org/content/early/2017/06/16/105064.abstract AB Accurate annotations of genes and their transcripts is a foundation of genomics, but no annotation technique presently combines throughput and accuracy. As a result, current reference gene collections remain far from complete: many genes models are fragmentary, while thousands more remain uncatalogued—particularly for long noncoding RNAs (lncRNAs). To accelerate lncRNA annotation, the GENCODE consortium has developed RNA Capture Long Seq (CLS), combining targeted RNA capture with third generation long-read sequencing. We present an experimental re-annotation of the entire GENCODE intergenic lncRNA population in matched human and mouse tissues. CLS approximately doubles the annotated complexity of targeted loci, in terms of validated splice junctions and transcript models, outperforming existing short-read techniques. The full-length transcript models produced by CLS enable us to definitively characterize the genomic features of lncRNAs, including promoter- and gene-structure, and protein-coding potential. Thus CLS removes a longstanding bottleneck of transcriptome annotation, generating manual-quality full-length transcript models at high-throughput scales.