@article {de Francisco Amorim150805, author = {Marcella de Francisco Amorim and Eva-Maria Willing and Emese Xochitl Szabo and Irina Droste-Borel and Boris Ma{\v c}ek and Korbinian Schneeberger and Sascha Laubinger}, title = {Arabidopsis U1 snRNP subunit LUC7 functions in alternative splicing and preferential removal of terminal introns}, elocation-id = {150805}, year = {2017}, doi = {10.1101/150805}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Introns are removed by the spliceosome, a large complex composed of five ribonucleoprotein subcomplexes (U snRNP). The U1 snRNP, which binds to 5{\textquoteright} splice sites, plays an essential role in early steps of the splicing reaction. Here, we show that Arabidopsis LUC7 is a U1 snRNP subunit that affects constitutive and alternative splicing. Interestingly, LUC7 specifically promotes splicing of a subset of terminal introns. Splicing of LUC7-dependent terminal introns is a prerequisite for nuclear export and can be modulated by stress. Globally, intron retention under stress conditions occurs preferentially among first and terminal introns, uncovering an unknown bias for splicing regulation in Arabidopsis. Taken together, our study reveals that the Arabidopsis U1 snRNP component LUC7 is important for alternative splicing and removal of terminal introns and it suggests that Arabidopsis terminal introns fine-tune gene expression under stress conditions.}, URL = {https://www.biorxiv.org/content/early/2017/06/16/150805}, eprint = {https://www.biorxiv.org/content/early/2017/06/16/150805.full.pdf}, journal = {bioRxiv} }