TY - JOUR T1 - Mapping the ecological networks of microbial communities from steady-state data JF - bioRxiv DO - 10.1101/150649 SP - 150649 AU - Yandong Xiao AU - Marco Tulio Angulo AU - Jonathan Friedman AU - Matthew K. Waldor AU - Scott T. Weiss AU - Yang-Yu Liu Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/06/15/150649.abstract N2 - Microbes form complex and dynamic ecosystems that play key roles in the health of the animals and plants with which they are associated. The inter-species interactions are often represented by a directed, signed and weighted ecological network, where nodes represent microbial species and edges represent ecological interactions. Inferring the underlying ecological networks of microbial communities is a necessary step towards understanding their assembly rules and predicting their dynamical response to external stimuli. However, current methods for inferring such networks require assuming a particular population dynamics model, which is typically not known a priori. Moreover, those methods require fitting longitudinal abundance data, which is not readily available, and often does not contain the variation that is necessary for reliable inference. To overcome these limitations, here we develop a new method to map the ecological networks of microbial communities using steady-state data. Our method can qualitatively infer the inter-species interaction types or signs (positive, negative or neutral) without assuming any particular population dynamics model. Additionally, when the population dynamics is assumed to follow the classic Generalized Lotka-Volterra model, our method can quantitatively infer the inter-species interaction strengths and intrinsic growth rates. We systematically validate our method using simulated data, and then apply it to experimental data from a synthetic soil microbial community. Our method offers a novel framework to infer microbial interactions and reconstruct ecological networks, and represents a key step towards reliable modeling of complex, real-world microbial communities, such as human gut microbiota. ER -