@article {Wen149245, author = {Jikai Wen and Laetitia Marzi and Jianming Wang and Jinxin Ye and Saverio Brogna}, title = {Splicing-dependent NMD requires Prp17 in Saccharomyces cerevisiae}, elocation-id = {149245}, year = {2017}, doi = {10.1101/149245}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Nonsense mediated mRNA decay (NMD) denotes that mutations, or errors in gene expression which introduce a premature translation termination codon (PTC), can decrease mRNA level in any organism. Remarkably, whether a PTC will lead to NMD often depends on the presence of a downstream intron, splicing of which marks a spliced mRNA by deposition of the exon junction complex (EJC) in mammalian cells. Saccharomyces cerevisiae, which has introns only in 5\% of its genes and lacks proteins essential for the EJC assembly was not expected to undergo splicing-dependent NMD. Conversely, here we report that the presence of an intron can enhance NMD in a distance-dependent manner regardless of whether it is positioned before or after a PTC in this organism. Following screening for genes that might be specifically involved in splicing-dependent NMD, we identified splicing factor Prp17. Our data indicate that the coupling between splicing and translation is a universal feature of eukaryotes, and envisage that Prp17, which, notably, co-sediments with monosomal mRNA, may link splicing to NMD by bridging the spliceosome with the ribosome.}, URL = {https://www.biorxiv.org/content/early/2017/06/12/149245}, eprint = {https://www.biorxiv.org/content/early/2017/06/12/149245.full.pdf}, journal = {bioRxiv} }