RT Journal Article
SR Electronic
T1 Multidimensional mapping of root responses to soil environmental cues using a luminescence-based imaging system
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 016931
DO 10.1101/016931
A1 Rubén Rellán-Álvarez
A1 Guillaume Lobet
A1 Heike Lindner
A1 Pierre-Luc Pradier
A1 Muh-Ching Yee
A1 Jose Sebastian
A1 Yu Geng
A1 Charlotte Trontin
A1 Therese LaRue
A1 Amanda Schrager-Lavelle
A1 Cara Haney
A1 Rita Nieu
A1 Julin Maloof
A1 John P. Vogel
A1 José R. Dinneny
YR 2015
UL http://biorxiv.org/content/early/2015/03/30/016931.abstract
AB Root systems develop different root types that individually sense eues from their local environment and integrate them with systemic signais. This complex multi-dimensional amalgam of inputs leads to continuous adjustment of root growth rates, direction and metabolic activity to define a dynamic physical network. Current methods for analyzing root biology balance physiological relevance with imaging capability. To bridge this divide, we developed an integrated imaging system called Growth and Luminescence Observatory for Roots (GLO-Roots) that uses luminescence-based reporters to enable studies of root architecture and gene expression patterns in soil-grown, light-shielded roots. We have developed image analysis algorithms that allow the spatial intgration of soil properties such as soil moisture with root traits. We propose GLO-Roots as a system that has great utility in both presenting environmental stimuli to roots in ways that evoke natural adaptive responses, and in providing tools for developing a multi-dimensional understanding of such processes.