PT  - JOURNAL ARTICLE
AU  - Abhishek Bhattacharya
AU  - Pranjal Biswas
AU  - Puranjoy Kar
AU  - Piya Roychoudhury
AU  - Sankar Basu
AU  - Sanjay Ghosh
AU  - Kaustab Panda
AU  - Ruma Pal
AU  - Anjan Kr. Dasgupta
TI  - Nitric oxide sensing by chlorophyll <em>a</em>
AID  - 10.1101/146563
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 146563
4099  - http://biorxiv.org/content/early/2017/06/06/146563.short
4100  - http://biorxiv.org/content/early/2017/06/06/146563.full
AB  - Nitric oxide (NO) acts as a signalling molecule that has direct and indirect regulatory roles in various functional processes in biology, though in plant kingdom its role is relatively unexplored. One reason for this is the fact that sensing of NO is always challenging. There are very few probes that can classify the different NO species. The present paper proposes a simple but straightforward way for sensing different NO species using chlorophyll, the source of inspiration being hemoglobin that serves as a NO sink in most mamalian system. The proposed method is able to classify NO from DETA-NONOate or (Z)-1-[N-(2-aminoethyl)-N-(2-ammonioethyl) amino] diazen-1-ium-1,2-diolate, nitrite, nitrate and S-nitrosothiol or SNO. This discrimination is carried out by chlorophyll a (chl a) at nano molar (nM) order of sensitivity and at 293K to 310K. Molecular docking reveals the differential binding behaviour of NO and SNO with chlorophyll, the predicted binding affinity matching with the experimental observation. Additional expreiments with diverse range of cyanobacteria reveals that apart from spectroscopic approach the proposed sensing module can be used in microscopic inspection of NO speices. Binding of NO is sensitive to tempertaure and static magnetic field. This provides additional support to the involvement of the porphyrin ring structure to the NO sensing process. This also broadens the scope of the sensing methods as hinted in the text.