PT - JOURNAL ARTICLE AU - Douglas H. Phanstiel AU - Kevin Van Bortle AU - Damek Spacek AU - Gaelen T. Hess AU - Muhammad Saad Shamim AU - Ido Machol AU - Michael I. Love AU - Erez Lieberman Aiden AU - Michael C. Bassik AU - Michael P. Snyder TI - Static and dynamic DNA loops form AP-1 bound activation hubs during macrophage development AID - 10.1101/142026 DP - 2017 Jan 01 TA - bioRxiv PG - 142026 4099 - http://biorxiv.org/content/early/2017/05/25/142026.short 4100 - http://biorxiv.org/content/early/2017/05/25/142026.full AB - The three-dimensional arrangement of the human genome comprises a complex network of structural and regulatory chromatin loops important for coordinating changes in transcription during human development. To better understand the mechanisms underlying context-specific 3D chromatin structure and transcription during cellular differentiation, we generated comprehensive in situ Hi-C maps of DNA loops during human monocyte-to-macrophage differentiation. We demonstrate that dynamic looping events are regulatory rather than structural in nature and uncover widespread coordination of dynamic enhancer activity at preformed and acquired DNA loops. Enhancer-bound loop formation and enhancer-activation of preformed loops represent two distinct modes of regulation that together form multi-loop activation hubs at key macrophage genes. Activation hubs connect 3.4 enhancers per promoter and exhibit a strong enrichment for Activator Protein 1 (AP-1) binding events, suggesting multi-loop activation hubs driven by cell-type specific transcription factors may represent an important class of regulatory chromatin structures for the spatiotemporal control of transcription.HIGHLIGHTSHigh resolution and high sensitivity of loop detection via deeply sequenced in situ Hi-C experiments during monocyte to macrophage differentiation (> 10 billion total reads)Multi-loop interaction communities identified surrounding key macrophage genes.Multi-loop communities connect dynamic enhancers through both static and newly acquired DNA loops, forming hubs of activationMacrophage activation hubs are enriched for AP-1 bound long-range enhancer interactions, suggesting cell-type specific TFs drive changes in 3D structure and transcription through regulatory DNA loops