RT Journal Article SR Electronic T1 Achieving better than 3 Å resolution by single particle cryo-EM at 200 keV JF bioRxiv FD Cold Spring Harbor Laboratory SP 141994 DO 10.1101/141994 A1 Mark A. Herzik, Jr. A1 Mengyu Wu A1 Gabriel C. Lander YR 2017 UL http://biorxiv.org/content/early/2017/05/25/141994.abstract AB Technical and methodological advances in single-particle cryo-electron microscopy (cryo-EM) have expanded the technique into a resolution regime that was previously only attainable by X-ray crystallography. Although single-particle cryo-EM has proven to be a useful technique for determining the structures of biomedically relevant molecules at near-atomic resolution, nearly 98% of the structures resolved to better than 4 Å resolution have been determined using 300 keV transmission electron microscopes (TEMs). We demonstrate that it is possible to obtain cryo-EM reconstructions of macromolecular complexes at a range of sizes to better than 3 Å resolution using a 200 keV TEM. These structures are of sufficient quality to unambiguously assign amino acid rotameric conformations and identify ordered water molecules, features previously thought only to be resolvable using TEMs operating at 300 keV.