TY - JOUR T1 - Aberration-corrected high-NA open-top selective-plane illumination microscopy for biological imaging JF - bioRxiv DO - 10.1101/140418 SP - 140418 AU - Ryan McGorty AU - Dan Xie AU - Bo Huang Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/05/20/140418.abstract N2 - Selective-plane illumination microscopy (SPIM) provides unparalleled advantages for volumetric imaging of living organisms over extended times. However, the spatial configuration of a SPIM system often limits its compatibility with many widely used biological sample holders such as multi-well chambers and plates. To solve this problem, we developed a high numerical aperture (NA) open-top configuration that places both the excitation and detection objectives on the opposite of the sample coverglass. We carried out a theoretical calculation to analyze the structure of the system-induced aberrations. We then experimentally showed aberration-correction using adaptive optics combined with static optical components, demonstrating neardiffraction-limited performance in imaging fluorescently labeled cells. ER -