TY - JOUR T1 - Identification of novel transcriptional regulators of PKA subunits in <em>Saccharomyces cerevisiae</em> by Quantitative Promoter–Reporter Screening JF - bioRxiv DO - 10.1101/015891 SP - 015891 AU - C Pautasso AU - K Chatfield-Reed AU - G Chua AU - V Zaremberg AU - S Rossi Y1 - 2015/01/01 UR - http://biorxiv.org/content/early/2015/03/01/015891.abstract N2 - The cAMP dependent protein kinase (PKA) signaling is a broad specificity pathway that plays important roles in the transduction of environmental signals triggering precise physiological responses. cAMP-signal transduction specificity is achieved and controlled at several levels. The Saccharomyces cereviciae PKA holoenzyme consists of two catalytic subunits encoded by TPK1, TPK2 and TPK3 genes, and two regulatory subunits encoded by BCY1 gene. In this work we studied the activity of these gene promoters using a reporter-synthetic genetic array screen, with the goal of identifying novel regulators of PKA subunits expression. Gene ontology (GO) analysis of the regulators identified showed that these regulators were enriched for annotations associated with roles in several GO biological process, as lipid and phosphate metabolism or transcription regulation and regulate all or some of the four promoters. Further characterization of the effect of these pathways on promoter activity and mRNA levels pointed to inositol, inositol polyphosphates, choline and phosphate as novel upstream signals that regulate transcription of PKA subunit genes. In addition, within each category there are genes that regulate only one of the promoters and genes that regulate more than one of them at the same time. These results support the role of transcription regulation of each PKA subunit in cAMP specificity signaling. Interestingly, many of the known targets of PKA phosphorylation are associated with the identified pathways, opening the possibility of a reciprocal regulation in which PKA would be coordinating different metabolic pathways and these processes would in turn, regulate expression of the kinase subunits. ER -