RT Journal Article SR Electronic T1 A complete bacterial genome assembled de novo using only nanopore sequencing data JF bioRxiv FD Cold Spring Harbor Laboratory SP 015552 DO 10.1101/015552 A1 Nicholas J. Loman A1 Joshua Quick A1 Jared T. Simpson YR 2015 UL http://biorxiv.org/content/early/2015/02/20/015552.abstract AB A method for de novo assembly of data from the Oxford Nanopore MinION instrument is presented which is able to reconstruct the sequence of an entire bacterial chromosome in a single contig. Initially, overlaps between nanopore reads are detected. Reads are then subjected to one or more rounds of error correction by a multiple alignment process employing partial order graphs. After correction, reads are assembled using the Celera assembler. We show that this method is able to assemble nanopore reads from Escherichia coli K-12 MG1655 into a single contig of length 4.6Mb permitting a full reconstruction of gene order. The resulting assembly has 98.4% nucleotide identity compared to the finished reference genome.