TY - JOUR T1 - A complete bacterial genome assembled <em>de novo</em> using only nanopore sequencing data JF - bioRxiv DO - 10.1101/015552 SP - 015552 AU - Nicholas J. Loman AU - Joshua Quick AU - Jared T. Simpson Y1 - 2015/01/01 UR - http://biorxiv.org/content/early/2015/02/20/015552.abstract N2 - A method for de novo assembly of data from the Oxford Nanopore MinION instrument is presented which is able to reconstruct the sequence of an entire bacterial chromosome in a single contig. Initially, overlaps between nanopore reads are detected. Reads are then subjected to one or more rounds of error correction by a multiple alignment process employing partial order graphs. After correction, reads are assembled using the Celera assembler. We show that this method is able to assemble nanopore reads from Escherichia coli K-12 MG1655 into a single contig of length 4.6Mb permitting a full reconstruction of gene order. The resulting assembly has 98.4% nucleotide identity compared to the finished reference genome. ER -