PT  - JOURNAL ARTICLE
AU  - Nicholas J. Loman
AU  - Joshua Quick
AU  - Jared T. Simpson
TI  - A complete bacterial genome assembled <em>de novo</em> using only nanopore sequencing data
AID  - 10.1101/015552
DP  - 2015 Jan 01
TA  - bioRxiv
PG  - 015552
4099  - http://biorxiv.org/content/early/2015/02/20/015552.short
4100  - http://biorxiv.org/content/early/2015/02/20/015552.full
AB  - A method for de novo assembly of data from the Oxford Nanopore MinION instrument is presented which is able to reconstruct the sequence of an entire bacterial chromosome in a single contig. Initially, overlaps between nanopore reads are detected. Reads are then subjected to one or more rounds of error correction by a multiple alignment process employing partial order graphs. After correction, reads are assembled using the Celera assembler. We show that this method is able to assemble nanopore reads from Escherichia coli K-12 MG1655 into a single contig of length 4.6Mb permitting a full reconstruction of gene order. The resulting assembly has 98.4% nucleotide identity compared to the finished reference genome.