%0 Journal Article %A Jason Merkin %A Ping Chen %A Maria Alexis %A Sampsa Hautaniemi %A Christopher B. Burge %T Origins and impacts of new mammalian exons %D 2015 %R 10.1101/009282 %J bioRxiv %P 009282 %X Mammalian genes are composed of exons, but the evolutionary origins and functions of new internal exons are poorly understood. Here, we analyzed patterns of exon gain using deep cDNA sequencing data from five mammals and one bird, identifying thousands of species- and lineage-specific exons. Most new exons derived from unique rather than repetitive intronic sequence. Unlike exons conserved across mammals, species-specific internal exons were mostly located in 5′ untranslated regions and alternatively spliced. They were associated with upstream intronic deletions, increased nucleosome occupancy, and RNA polymerase II pausing. Genes containing new internal exons had increased gene expression, but only in tissues where the exon was included. Increased expression correlated with level of exon inclusion, promoter proximity, and signatures of cotranscriptional splicing. Together these findings suggest that splicing at the 5′ ends of genes enhances expression and that changes in 5′ end splicing alter gene expression between tissues and between species. %U https://www.biorxiv.org/content/biorxiv/early/2015/02/18/009282.full.pdf