RT Journal Article
SR Electronic
T1 Three-dimensional Two-Photon Optogenetics and Imaging of Neural Circuits <em>in vivo</em>
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 132506
DO 10.1101/132506
A1 Weijian Yang
A1 Luis Carrillo-Reid
A1 Yuki Bando
A1 Darcy S. Peterka
A1 Rafael Yuste
YR 2017
UL http://biorxiv.org/content/early/2017/04/30/132506.abstract
AB We demonstrate a holographic system for simultaneous three-dimensional (3D) two-photon stimulation and imaging of neural activity in the mouse neocortex in vivo with cellular resolution. Dual two-photon excitation paths are implemented with independent 3D targeting for calcium imaging and precision optogenetics. We validate the usefulness of the microscope by photoactivating local pools of interneurons in awake mice visual cortex in 3D, which suppress the nearby pyramidal neurons’ response to visual stimuli.