RT Journal Article
SR Electronic
T1 A practical guide to CRISPR/Cas9 genome editing in Lepidoptera
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 130344
DO 10.1101/130344
A1 Linlin Zhang
A1 Robert D. Reed
YR 2017
UL http://biorxiv.org/content/early/2017/04/25/130344.abstract
AB CRISPR/Cas9 genome editing has revolutionized functional genetic work in many organisms and is having an especially strong impact in emerging model systems. Here we summarize recent advances in applying CRISPR/Cas9 methods in Lepidoptera, with a focus on providing practical advice on the entire process of genome editing from experimental design through to genotyping. We also describe successful targeted GFP knock-ins that we have achieved in butterflies. Finally, we provide a complete, detailed protocol for producing targeted long deletions in butterflies.