RT Journal Article
SR Electronic
T1 Genome sequencing reveals Zika virus diversity and spread in the Americas
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 109348
DO 10.1101/109348
A1 Metsky H.C.
A1 Matranga C.B.
A1 Wohl S.
A1 Schaffner S.F.
A1 Freije C.A.
A1 Winnicki S.M.
A1 West K.
A1 Qu J.
A1 Baniecki M.L.
A1 Gladden-Young A.
A1 Lin A.E.
A1 Tomkins-Tinch C.H.
A1 Ye S.H.
A1 Park D.J.
A1 Luo C.Y.
A1 Barnes K.G.
A1 Shah R.R.
A1 Chak B.
A1 Barbosa-Lima G.
A1 Delatorre E.
A1 Vieira Y.R.
A1 Paul L.M.
A1 Tan A.L.
A1 Barcellona C.M.
A1 Porcelli M.C.
A1 Vasquez C.
A1 Cannons A.C.
A1 Cone M.R.
A1 Hogan K.N.
A1 Kopp E.W.
A1 Anzinger J.J.
A1 Garcia K.F.
A1 Parham L.A.
A1 Gélvez Ramírez R.M.
A1 Miranda Montoya M.C.
A1 Rojas D.P.
A1 Brown C.M.
A1 Hennigan S.
A1 Sabina B.
A1 Scotland S.
A1 Gangavarapu K.
A1 Grubaugh N.D.
A1 Oliveira G.
A1 Robles-Sikisaka R.
A1 Rambaut A.
A1 Gehrke L.
A1 Smole S.
A1 Halloran M.E.
A1 Villar Centeno L.A.
A1 Mattar S.
A1 Lorenzana I.
A1 Cerbino-Neto J.
A1 Valim C.
A1 Degrave W.
A1 Bozza P.T.
A1 Gnirke A.
A1 Andersen K.G.
A1 Isern S.
A1 Michael S.F.
A1 Bozza F.A.
A1 Souza T.M.L.
A1 Bosch I.
A1 Yozwiak N.L.
A1 MacInnis B.L.
A1 Sabeti P.C.
YR 2017
UL http://biorxiv.org/content/early/2017/04/23/109348.abstract
AB Despite great attention given to the recent Zika virus (ZIKV) epidemic in the Americas, much remains unknown about its epidemiology and evolution, in part due to a lack of genomic data. We applied multiple sequencing approaches to generate 110 ZIKV genomes from clinical and mosquito samples from 10 countries and territories, greatly expanding the observed viral genetic diversity from this outbreak. We analyzed the timing and patterns of introductions into distinct geographic regions; our phylogenetic evidence suggests rapid expansion of the outbreak in Brazil and multiple introductions of outbreak strains into Puerto Rico, Honduras, Colombia, other Caribbean islands, and the continental US. We find that ZIKV circulated undetected in multiple regions for many months before the first locally transmitted cases were confirmed, highlighting the importance of viral surveillance. We identify mutations with possible functional implications for ZIKV biology and pathogenesis, as well as those potentially relevant to the effectiveness of diagnostic tests.