PT - JOURNAL ARTICLE AU - C Zhu AU - Y Mahlich AU - M Miller AU - Y Bromberg TI - <em>fusion</em>DB: assessing microbial diversity and environmental preferences via functional similarity networks AID - 10.1101/035923 DP - 2017 Jan 01 TA - bioRxiv PG - 035923 4099 - http://biorxiv.org/content/early/2017/04/20/035923.short 4100 - http://biorxiv.org/content/early/2017/04/20/035923.full AB - Microbial functional diversification is driven by environmental factors, i.e. microorganisms inhabiting the same environmental niche tend to be more functionally similar than those from different environments. In some cases, even closely phylogenetically related microbes differ more across environments than across taxa. While microbial similarities are often reported in terms of taxonomic relationships, no existing databases directly links microbial functions to the environment. We previously developed a method for comparing microbial functional similarities on the basis of proteins translated from the sequenced genomes. Here we describe fusionDB, a novel database that uses our functional data to represent 1,374 taxonomically distinct bacteria annotated with available metadata: habitat/niche, preferred temperature, and oxygen use. Each microbe is encoded as a set of functions represented by its proteome and individual microbes are connected via common functions. Users can search fusionDB via combinations of organism names and metadata. Moreover, the web interface allows mapping new microbial genomes to the functional spectrum of reference bacteria, rendering interactive similarity networks that highlight shared functionality. fusionDB provides a fast means of comparing microbes, identifying potential horizontal gene transfer events, and highlighting key environment-specific functionality.fusionDB is publicly available at http://services.bromberglab.org/fusiondb/.