RT Journal Article
SR Electronic
T1 STRT-seq-2i: dual-index 5′ single cell and nucleus RNA-seq on an addressable microwell array
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 126268
DO 10.1101/126268
A1 Hannah Hochgerner
A1 Peter Lönnerberg
A1 Rebecca Hodge
A1 Jaromir Mikes
A1 Abeer Heskol
A1 Hermann Hubschle
A1 Philip Lin
A1 Simone Picelli
A1 Gioele La Manno
A1 Michael Ratz
A1 Jude Dunne
A1 Syed Husain
A1 Ed Lein
A1 Maithreyan Srinivasan
A1 Amit Zeisel
A1 Sten Linnarsson
YR 2017
UL http://biorxiv.org/content/early/2017/04/20/126268.abstract
AB Single-cell RNA-seq has become routine for discovering cell types and revealing cellular diversity, but currently no high-throughput platform has been used successfully on archived human brain samples. We present STRT-seq-2i, an addressable 9600-microwell array platform, combining sampling by limiting dilution or FACS, with imaging and high throughput at competitive cost. We applied the platform to fresh single mouse cortical cells and to frozen post-mortem human cortical nuclei, matching the performance of a previous lower-throughput platform.