RT Journal Article
SR Electronic
T1 Rapid Sensing of Volumetric Neural Activity through Adaptive Remote Focusing
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 125070
DO 10.1101/125070
A1 Mantas Žurauskas
A1 Oliver Barnstedt
A1 Maria Frade-Rodriguez
A1 Scott Waddell
A1 Martin J. Booth
YR 2017
UL http://biorxiv.org/content/early/2017/04/20/125070.abstract
AB The ability to record neural activity in the brain of a living organism at cellular resolution is of great importance for defining the neural circuit mechanisms that direct behavior. Here we present an adaptive two-photon microscope optimized for extraction of neural signals over volumes in intact Drosophila brains, even in the presence of specimen motion. High speed volume imaging was made possible through reduction of spatial resolution while maintaining the light collection efficiency of a high resolution, high numerical aperture microscope. This enabled simultaneous recording of odor-evoked calcium transients in a defined volume of mushroom body Kenyon cell bodies in a live fruit fly.