TY - JOUR T1 - Rapid Sensing of Volumetric Neural Activity through Adaptive Remote Focusing JF - bioRxiv DO - 10.1101/125070 SP - 125070 AU - Mantas Žurauskas AU - Oliver Barnstedt AU - Maria Frade-Rodriguez AU - Scott Waddell AU - Martin J. Booth Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/04/20/125070.abstract N2 - The ability to record neural activity in the brain of a living organism at cellular resolution is of great importance for defining the neural circuit mechanisms that direct behavior. Here we present an adaptive two-photon microscope optimized for extraction of neural signals over volumes in intact Drosophila brains, even in the presence of specimen motion. High speed volume imaging was made possible through reduction of spatial resolution while maintaining the light collection efficiency of a high resolution, high numerical aperture microscope. This enabled simultaneous recording of odor-evoked calcium transients in a defined volume of mushroom body Kenyon cell bodies in a live fruit fly. ER -