RT Journal Article
SR Electronic
T1 Slingshot: Cell lineage and pseudotime inference for single-cell transcriptomics
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 128843
DO 10.1101/128843
A1 Kelly Street
A1 Davide Risso
A1 Russell B. Fletcher
A1 Diya Das
A1 John Ngai
A1 Nir Yosef
A1 Elizabeth Purdom
A1 Sandrine Dudoit
YR 2017
UL http://biorxiv.org/content/early/2017/04/19/128843.abstract
AB Single-cell transcriptomics allows researchers to investigate complex communities of heterogeneous cells. These methods can be applied to stem cells and their descendants in order to chart the progression from multipotent progenitors to fully differentiated cells. While a number of statistical and computational methods have been proposed for analyzing cell lineages, the problem of accurately characterizing multiple branching lineages remains difficult to solve. Here, we introduce a novel method, Slingshot, for inferring multiple developmental lineages from single-cell gene expression data. Slingshot is a uniquely robust and flexible tool for inferring developmental lineages and ordering cells to reflect continuous, branching processes.