PT  - JOURNAL ARTICLE
AU  - Kelly Street
AU  - Davide Risso
AU  - Russell B. Fletcher
AU  - Diya Das
AU  - John Ngai
AU  - Nir Yosef
AU  - Elizabeth Purdom
AU  - Sandrine Dudoit
TI  - Slingshot: Cell lineage and pseudotime inference for single-cell transcriptomics
AID  - 10.1101/128843
DP  - 2017 Jan 01
TA  - bioRxiv
PG  - 128843
4099  - http://biorxiv.org/content/early/2017/04/19/128843.short
4100  - http://biorxiv.org/content/early/2017/04/19/128843.full
AB  - Single-cell transcriptomics allows researchers to investigate complex communities of heterogeneous cells. These methods can be applied to stem cells and their descendants in order to chart the progression from multipotent progenitors to fully differentiated cells. While a number of statistical and computational methods have been proposed for analyzing cell lineages, the problem of accurately characterizing multiple branching lineages remains difficult to solve. Here, we introduce a novel method, Slingshot, for inferring multiple developmental lineages from single-cell gene expression data. Slingshot is a uniquely robust and flexible tool for inferring developmental lineages and ordering cells to reflect continuous, branching processes.