%0 Journal Article %A Konstantin A. Blagodatskikh %A Vladimir M. Kramarov %A Ekaterina V. Barsova %A Alexey V. Garkovenko %A Dmitriy S. Shcherbo %A Andrew A. Shelenkov %A Vera V. Ustinova %A Maria R. Tokarenko %A Simon C. Baker %A Tatiana V. Kramarova %A Konstantin B. Ignatov %T Improved DOP-PCR (iDOP-PCR): a robust and simple WGA method for efficient amplification of low copy number genomic DNA %D 2017 %R 10.1101/128736 %J bioRxiv %P 128736 %X Whole-genome amplification (WGA) techniques are used for non-specific amplification of low-copy number DNA, and especially for single-cell genome and transcriptome amplification. There are a number of WGA methods that have been developed over the years. One example is degenerate oligonucleotide-primed PCR (DOP-PCR), which is a very simple, fast and inexpensive WGA technique. Although DOP-PCR has been regarded as one of the pioneering methods for WGA, it only provides low genome coverage and a high allele dropout rate when compared to more modern techniques. Here we describe an improved DOP-PCR (iDOP-PCR). We have modified the classic DOP-PCR by using a new thermostable DNA polymerase (SD polymerase) with a strong strand-displacement activity and by adjustments in primers design. We compared iDOP-PCR, classic DOP-PCR and the well-established PicoPlex technique for whole genome amplification of both high- and low-copy number human genomic DNA. The amplified DNA libraries were evaluated by analysis of short tandem repeat genotypes and NGS data. In summary, iDOP-PCR provided a better quality of the amplified DNA libraries compared to the other WGA methods tested, especially when low amounts of genomic DNA were used as an input material. %U https://www.biorxiv.org/content/biorxiv/early/2017/04/19/128736.full.pdf