RT Journal Article
SR Electronic
T1 A multi-model approach to assessing local and global cryo-EM map quality
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 128561
DO 10.1101/128561
A1 Mark A. Herzik, Jr.
A1 James S. Fraser
A1 Gabriel C. Lander
YR 2017
UL http://biorxiv.org/content/early/2017/04/19/128561.abstract
AB There does not currently exist a standardized indicator of how well a cryo-EM-derived model represents the density from which it was generated. We present a straightforward methodology that utilizes freely available tools to generate a suite of independent models and to evaluate their convergence in an EM density. These analyses provide both a quantitative and qualitative assessment of the precision of the models and their representation of the density, respectively, while concurrently providing a platform for assessing both global and local EM map quality. We further use standardized datasets to provide an expected model–model agreement criterion for EM maps reported to be at 5 Å resolution or better. Associating multiple atomic models with a deposited EM map provides a rapid and accessible reporter of convergence, a strong indicator of highly resolved molecular detail, and is an important step toward an FSC-independent assessment of map and model quality.