RT Journal Article SR Electronic T1 Simultaneous Monitoring of Behavior & Peripheral Clocks in Drosophila Reveals Unstructured Sleep in an Alzheimer’s Model JF bioRxiv FD Cold Spring Harbor Laboratory SP 013730 DO 10.1101/013730 A1 Eleonora Khabirova A1 Ko-Fan Chen A1 John S. O’Neill A1 Damian C. Crowther YR 2015 UL http://biorxiv.org/content/early/2015/01/31/013730.abstract AB Sleep and circadian rhythms are ancient, related phenomena controlled by distinct circuitry, whose appropriate regulation is critical to health. The regulatory machinery underlying sleep homeostasis is ill-defined, but biological clock mechanisms are better understood: from ‘clock gene’ oscillations to rest/activity cycles. Age- and neurodegeneration-related deterioration in sleep/wake timing was first described in humans decades ago, and has recently been recapitulated in model organisms. To delineate causal relationships between aging, sleep, neuronal function and the molecular clockwork, we developed FLYGLOW, a bioluminescence-based system which allows rest/activity, sleep consolidation and molecular rhythms to be quantified simultaneously in many individual flies over days/weeks. FLYGLOW outperforms existing methods, and allows multiparameter correlational analyses within and between flies. We also show unambiguously that peripheral tissue rhythms free-run independently of the central pacemakers driving behavioural cycles. Using an Alzheimer’s fly model we observe a profound disorganization of sleep/wake cycles, phenocopying the human disease.