RT Journal Article
SR Electronic
T1 Autonomous combinatorial color barcoding for multiplexing single molecule RNA visualization
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 127373
DO 10.1101/127373
A1 Yong-Sheng Cheng
A1 Yue Zhuo
A1 Katharina Hartmann
A1 Peng Zou
A1 Gözde Bekki
A1 Heike Alter
A1 Hai-Kun Liu
YR 2017
UL http://biorxiv.org/content/early/2017/04/18/127373.abstract
AB Single molecular fluorescence in situ hybridization (smFISH) detects RNA transcripts with spatial information and digital molecular counting. However, the broad usage of smFISH is still hindered by the complex chemical probe conjugation or microscopy set-up, especially for investigating multiple gene expression. Here we present a multiple fluorophore enzymatic labeling method (termed HuluFISH) for smFISH probes to achieve flexible combinatorial color barcoding in single hybridization step. The multiplex capacity of HuluFISH follows an exponential growth with the increase of the number of fluorophore types. We demonstrate that this method can be used to detect cellular heterogeneity in embryonic mouse brain on single cell level.