TY - JOUR T1 - Autonomous combinatorial color barcoding for multiplexing single molecule RNA visualization JF - bioRxiv DO - 10.1101/127373 SP - 127373 AU - Yong-Sheng Cheng AU - Yue Zhuo AU - Katharina Hartmann AU - Peng Zou AU - Gözde Bekki AU - Heike Alter AU - Hai-Kun Liu Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/04/18/127373.abstract N2 - Single molecular fluorescence in situ hybridization (smFISH) detects RNA transcripts with spatial information and digital molecular counting. However, the broad usage of smFISH is still hindered by the complex chemical probe conjugation or microscopy set-up, especially for investigating multiple gene expression. Here we present a multiple fluorophore enzymatic labeling method (termed HuluFISH) for smFISH probes to achieve flexible combinatorial color barcoding in single hybridization step. The multiplex capacity of HuluFISH follows an exponential growth with the increase of the number of fluorophore types. We demonstrate that this method can be used to detect cellular heterogeneity in embryonic mouse brain on single cell level. ER -