@article {Haapasalo127514, author = {Karita Haapasalo and Adam J.M Wollman and Carla de Haas and Kok van Kessel and Jos van Strijp and Mark C. Leake}, title = {Characterization of the stoichiometry of the complex formed by Staphylococcal LukSF and human C5aR receptor in living cells}, elocation-id = {127514}, year = {2017}, doi = {10.1101/127514}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Staphylococcus aureus Panton Valentine Leukocidin (PVL) is a pore-forming toxin comprising protein subunits LukS and LukF. Binding of LukS to human C5a receptor (hC5aR) on leukocytes induces secondary binding of LukF and assembly of lytic complexes. Previous analysis suggests that PVL consists of 4-plus-4 LukS/LukF subunits but the exact stoichiometry between LukS, LukF and hC5aR is not yet known. In this study we determine the stoichiometry and spatiotemporal dynamics of functional LukS/LukF-hC5aR complexes in living eukaryotic cells. By using rapid total internal reflection fluorescence (TIRF) and single-molecule photobleaching analysis we found that tetrameric LukS-hC5aR complexes are formed within a cluster of receptors. Upon binding to hC5aR each LukS subunit binds LukF leading to lytic pore formation and simultaneous dissociation of receptors from the complex. Our findings corroborate a hetero-octamer model but provide a new view on the kinetics of crucial virulence factor assembly on integrated host cell membrane receptors.}, URL = {https://www.biorxiv.org/content/early/2017/04/14/127514}, eprint = {https://www.biorxiv.org/content/early/2017/04/14/127514.full.pdf}, journal = {bioRxiv} }