TY - JOUR T1 - Cell type boundaries organize plant development JF - bioRxiv DO - 10.1101/126466 SP - 126466 AU - Monica Pia Caggiano AU - Xiulian Yu AU - Neha Bhatia AU - André Larsson AU - Hasthi Ram AU - Carolyn Ohno AU - Pia Sappl AU - Elliot M. Meyerowitz AU - Henrik Jönsson AU - Marcus G. Heisler Y1 - 2017/01/01 UR - http://biorxiv.org/content/early/2017/04/12/126466.abstract N2 - Once sentence summary Cell type boundaries regulate plant developmentAbstract In plants the dorsoventral boundary of leaves defines an axis of symmetry through the centre of the organ separating the future top (dorsal) and bottom (ventral) tissues. Although the positioning of this boundary is critical for leaf morphogenesis, the mechanism by which it is established has not been determined. Here we show that dorsoventral boundaries in leaves derive from a boundary between Class III homeodomain-leucine zipper and KANADI expression in the shoot apical meristem, confirming that leaf founder cells are pre-patterned with respect to their dorsoventral axis. Furthermore, perturbation of this boundary not only alters leaf orientation and morphogenesis but also organ position, revealing a general role for these boundaries in plant development. Lastly, we show that auxin stabilizes boundary position at organ inception and that wounds disrupt this process. Overall our findings reveal a two-way interaction between cell type boundaries and auxin that controls fundamental aspects of plant architecture. ER -